Product Specifications: Item# 2009: Recombinant HIV-2 (Rod) gp36 Envelope Protein (E.coli) Concentration: See vial Mass/vial: 0.1mg Volume/vial: 0.1ml Diluent: 10 mM Tris, pH 8.0, SDS (98% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 24 months at -75°C. Application: Human Diagnostics, Immunization,T-Cell Activation. Description: Truncated Recombinant HIV-2 envelope protein gp36 produced as a fusion protein in the E.coli Expression System Molecular Weight: 28kD Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced. Specificity: This protein binds to murine anti-gp36 HIV-2 monoclonal antibodies and HIV-2 converted human serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: ND Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.
