PRODUCT SPECIFICATIONS ITEM# 7003 : Recombinant Human APOBEC3B (E.coli) SPECIFICATIONS APPLICATIONS Concentration: See vial Vif binding assays Mass/vial: 100ug RNA binding assays Diluent: PBS, 0.1% Sarcosyl Purity: >95% Stabilizer: None Preservative: None Storage: -75oC Physical State: Frozen Liquid Stability: 12 Months at -85C DESCRIPTION: Produced in the E.coli Expression System as a hexa-His fusion protein. PURIFICATION: This protein is purified by Ni-affinity and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced. Approx MW: 46kD. SPECIFICITY: This protein binds to anti-CEM15 murine monoclonal antibodies and rabbit polyclonal antibodies as determined by Elisa and Western Elisa. BIOLOGICAL ACTIVITY: Binds to recombinant vif as determined by 3B-vif elisa assays using murine anti-vif mAb HRP as a secondary reagent.
