Product Specifications: Item# 7001 Recombinant Human T-Cell Receptor sCD4 Concentration: See vial Mass/vial: 0.1mg Volume/vial: 1ml Diluent: PBS Purity: >98% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 24 months at -75°C Applications: T-Cell Research,Human Diagnostics, HIV-1 Neutralization, Drug Screening Description: Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4. Purification:This protein is purified by immunoaffinity and gel exclusion chromatography to >98% purity as determined by SDS PAGE, reduced. Molecular weight: Approximately 55kD. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA. Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs. Application and Instructions for use: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s). 2. Recommended concentration for virus neutralization assays are in 1-10µg/ml of the HIV-1 infected culture. 3. Drug screening assays are performed with 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate (catalog# 1121-P) or directly using HIV-1 rgp120-peroxidase conjugate (catalog# 1001-P).
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