PRODUCT# 105-40: Recombinant M. tuberculosis MTP-64 Antigen (E.coli) SPECIFICATIONS APPLICATIONS Concentration: see vial ELISA Mass/vial: see vial Western ELISA Diluent: 50mMTris, 0.1M Nacl, pH 8.5 TB Diagnostics Purity: >98% Immunization Stabilizer: None Preservative: None Storage: -75C Physical State: Frozen Liquid Stability: 24 months at 75oC. DESCRIPTION: M. tuberculosis (virulent strain) MTP-64 antigen – synthetic gene -produced in the E. coli expression system. PURIFICATION: Purified by His Select affinity chromatography to >98% purity as determined by SDS-PAGE, reduced. MW: approx. 33.9kD SPECIFICITY: This MTP-64 protein reacts with M. tuberculosis converted human serum in ELISA and Western ELISA. Suitable for diagnostic applications. BIOLOGICAL ACTIVITY: Not done APPLICATION AND INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.
