.rte-operation-img { padding: 10px; margin: 5px 5px 25px; border-radius: 12px; box-shadow: 0 0 5px rgba(22, 47, 101, 0.3); display: flex; justify-content: center; overflow: hidden; } .rte-operation-img img { width: auto; max-height: 500px; } .rte-hr { background: linear-gradient(to right, white, #162F65); border: 0; height: 1px; } .operation-alert { background-color: #ffecd9; border-radius: 8px; padding: 10px; margin: 0 5px; display: flex; gap: 10px; color: #c62828; box-shadow: 0 0 5px rgba(22, 47, 101, 0.3); margin-bottom: 20px; } .operation-alert-icon { margin-top: 3px; } Product Information Ribonuclease R (RNase R) from E. coli, is a magnesium-dependent 3' to 5' exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures, or double-stranded RNA with 3'-overhangs shorter than 7 nucleotides. RNase R digests RNA from the 3' to 5' direction, releasing 5'-monophosphate nucleotides to produce dinucleotide and trinucleotide end products. Resource: Recombinant E.coliConcentration: 20U/μLUnit Definition: One unit converts 1μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37℃.Storage Buffer: 50mM Tris-HCl, 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v/v)Triton X-100, 50%(v/v) glycerol, pH7.5@25℃. Key points of operation T4 RNA ligase 1, T4 RNA ligase 2, RNaseR on enzymatic synthesis of circular RNA: IVT reaction to obtain linear RNA: it is worth noting that for this step of IVT reaction, a certain proportion of GMP needs to be doped to obtain GMP-containing linear RNA; RNA cyclisation: circular RNA is produced by joining the two ends within the RNA molecule by T4 RNA ligase 1 (T4 Rnl1) or T4 RNA ligase 2 (T4 Rnl2) catalysing the joining of 5'-phosphate and 3'-hydroxyl groups at the RNA terminus; Removal of linear RNA: RNaseR is able to remove all linear RNA molecules from the reaction solution, resulting in linear RNA-free circular RNA. Security Information Storage Conditions: Stored at -20±5°C. Do not store in a frost-free freezer. Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability. Quality Assurance: Functional Assay: Passes the internal function testing. Contaminant Assay: Free of detectable Endonuclease and Exonuclease. Endotoxin Assay: Pass. Physical Purity: >95% by SDS-PAGE with Coomassie® blue staining.
