Product Specifications: Item# 5008: Recombinant SIVmac251 nef (Baculovirus) Concentration: See vial Mass/vial: 100µg Diluent: 50mM Tris, pH8, 150mM NaCl Purity : >95% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 6 months at -75°C. Applications: Cell Activation,Immunological Studies, Drug Screening, ELISA Western ELISA, Diagnostics Description: Full length Recombinant SIV mac251 nef produced as a GST fusion protein in the Baculovirus Expression System. Purification: This recombinant protein is purified by Glutathione -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced. Specificity: This protein shows reactivity with murine monoclonal antibodies and SIV converted serum polyclonal antibodies in ELISA and Western ELISA. Biological activity: Not done. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.
