The Roche LightCycler 2.0 is a premier carousel-based real-time PCR system designed for rapid, high-precision nucleic acid amplification and analysis. Known for its exceptional thermal cycling speed and accuracy, the 2.0 system utilizes high-quality glass capillaries that provide an optimal surface-to-volume ratio for ultra-fast heat transfer. This system is a versatile "workhorse" for laboratories performing qualitative and quantitative detection, genotyping, and mutation analysis across clinical and research disciplines. Technical Performance and Design Rapid Thermal Cycling: Capable of completing a full 35-cycle PCR run in as little as 30 minutes, significantly increasing laboratory throughput compared to traditional block-based cyclers. Six-Channel Multiplexing: Features six dedicated detection channels (530, 560, 610, 640, 670, and 710 nm), enabling advanced multiplex experiments and the simultaneous monitoring of up to 32 PCR products. Superior Heat Transfer: Uses forced-air heating and cooling combined with specialized glass capillaries to ensure uniform temperature distribution and rapid ramp rates of up to 20°C per second. Online Kinetic Monitoring: The LightCycler Software 4.1 provides real-time data visualization, allowing researchers to monitor amplification progress and terminate runs early if sufficient signal is achieved. Product Specifications Model: LightCycler 2.0 Sample Capacity: 32-position carousel Reaction Vessels: 20 microliter or 100 microliter glass capillaries Excitation Source: High-intensity Blue LED (peak at 470 nm) Detection Channels: 530, 560, 610, 640, 670, 710 nm Temperature Accuracy: ± 0.4°C Dimensions: 11 x 15 x 20 inches (28 x 38.5 x 50.5 cm) Weight: Approximately 48.5 lbs (22 kg) Operational Advantages Flexible Fluorescence Formats: Compatible with a wide variety of dyes and probes, including SYBR Green I, HybProbe, and Hydrolysis (TaqMan) probes for diverse experimental designs. Minimized Contamination Risk: The sealed capillary system reduces the risk of amplicon carry-over, ensuring the integrity of the laboratory environment and sample results. Broad Dynamic Range: Provides sensitive detection and precise quantification over a wide range of target concentrations, from single copies up to millions of molecules. Comprehensive Analysis Modules: The integrated software supports complex data processing, including absolute and relative quantification, melting curve genotyping, and T-m calling for mutation screening. Used; excellent condition.
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