PRODUCT SPECIFICATIONS ITEM# 4003: Recombinant HCV 20kD Core Protein (Pichia) SPECIFICATIONS APPLICATIONS Concentration: 500ug/ml In-Vitro HCV Diagnostics Mass/vial: 100ug T-Cell Activation Volume/vial: 200ul Diluent: 20mM Citrate-Phosphate, pH6.0, 0.5M Urea Purity : >90% Stabilizer: None Preservative: None Storage: -75o C Stability: At least 6 months at -75o C DESCRIPTION: Full length HCV p22 capsid his-tag protein expressed in the Pichia pastori Expression System. PURIFICATION: HCV core fusion protein is purified by Ni-affinity chromatography methods to > 90% purity as determined by SDS-PAGE, reduced. SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and HCV converted human serum polyclonal antibodies in ELISA and Western ELISA. BIOLOGICAL ACTIVITY: Not determined. APPLICATION AND INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 50 to 100-fold in suitable buffer for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. HCV core protein shows relatively strong biding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.
