Simmons Citrate Agar Simmons Citrate Agar is used as a differential medium for the identification of enteric bacteria by using Citrate as the sole source of carbon and Ammonium as the sole source of nitrogen. Koser developed a fluid medium to determine citrate utilization, with Simmon further modifying the formula by adding 1.5% agar and Bromthymol Blue as an indicator. The medium can be used to distinguish between Escherichia coli and Enterobacter aerogenes and to assist in differentiating members of the Salmonella genus. Organisms able to metabolize the citrate (most Salmonella spp., some Enterobacteriaceae, most Klebsiella spp.) grow well on the surface of the medium and alkalinize the medium, changing it from green to blue. In turn, non-citrate fermenters (E. coli, Shigella spp.) are unable to shift the color. Simmons Citrate Agar is available in three sizes - 500g, 2kg, and 10kg. PREPARATION: Mix 24.2 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Autoclave at 121°C for 15 minutes. After autoclaving, place medium on a slant and allow for solidification. Additional Literature: S19-111 Product Information S19-111 Safety Data Sheet
