Sindbis (TR339)/Eastern Equine Encephalitis (FL93-939) Chimeric Virus

Advanced Virology Inc. Verified independent store

→

Description: This virus is a chimeric alphavirus containing the non-structural proteins of Sindbis virus (SINV) strain TR339 and the structural proteins from Eastern equine encepha litis virus (EEEV) strain FL93-939. This product allows the study of EEEV FL93-939 in a non-Select Agent BSL-2 laboratory setting.Strain History: TR339 is the consensus wild-type strain of SINV derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI. EEEV strain FL93-939 was isolated from an infected mosquito pool (Culiseta melanura) in Florida in 1993. The genomic sequence is available in NCBI.Passage History: SINV TR339: Suckling mouse brain (8). References (1) and (2). EEEV FL93-939: Vero (Unknown), newborn mouse brain (1). References (3) and (4). Production: Transfection of in vitro transcribed viral genomic RNA into baby hamster kidney (BHK) cells. No serial passages performed.Biosafety Level: 2Titer: 1.78 x 109 PFU/mL on BHK cells.Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.QA/QC: Titer in PFU/mL by plaque assay Genome equivalents/mL by qPCR Whole-genome sequencing by NGS Mycoplasma test by PCR Sterility test for bacterial and fungal contamination Endotoxin test Show more