IVC Medium For in vitro culture of embryos Description and Prod. No. 20 ml IVC medium in glass bottle, Prod. No. 5.07.020 The medium is a one-step medium that can be used without change of medium for the entire period from inseminated oocytes to the blastocyst stages Suitable for culture in 4WP dishes with 500 μl/well or in 100 μl drops Use Stroebech Oil as overlay to avoid evaporation. The medium is Sodium Bicarbonate buffered and requires CO2 equilibration The medium is ready-to-use and does not need any supplements. Medium should be preheated to 38.8°C prior to use Key Ingredients: Insulin, Hyaluronic Acid, EDTA, Calcium L-Lactate, Sodium Selenite, L-Carnitine, Ethanolamine, Sodium Pyruvate, Albumin, Transferrin, Myo-Inositol, Zink Sulphate and other Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, Ala-Glutamine, Glucose, Phenol Red, and Gentamycin Quality Control Tests and Manufacturing Sterility tested (Fungal and Bacterial) Osmolality tested and pH tested Endotoxin tested Manufacturer is ISO9001 and ISO13485 certified Controlled environment and to human standards Bovine Embryo Assay (BEA) Batch analysis certificate available Storage: Store in original unopened container refrigerated 3-9 °C and protected from light Do not expose to temperatures higher than 39°C Do not freeze product it may precipitate < 2°C The medium must be used within 10 days after opening, providing it has been handled aseptically and is kept refrigerated after opening Discard unused medium if it has been warmed
