Specification Package 30 RXNS Full name SpeedyCut Esp3I(BsmBI) Applications Fast enzymatic digestion Summary SpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps. Features All SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors. Composition 30 ul enzyme, 10X SpeedyOne Buffer Recognition Site 5'…-CGTCTC(N)1↓…-3' 3'…-GCAGAG(N)5↑…-5' Reaction Buffer 10X SpeedyOne Buffer Recommended Reaction Temperature 37°C Unit Definition At 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes. Methylation Effects Dam: Methylation does not affect; Dcm: Methylation does not affect; CpG: When the sequences overlap completely, cleavage is blocked; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage may be blocked. Thermal Inactivation Heat at 80 °C for 20 min to lose activity. Component 30 ul enzyme, 10X SpeedyOne Buffer Storage -20°C Documents Manual MSDS
