Specification Package 200 RXNS Full name SpeedyCut TaqI Applications Fast enzymatic digestion Summary SpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps. Features All SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors. Composition 200 ul enzyme, 10X SpeedyOne buffer Recognition Site 5'…-T↓CGA-…3' 3'…-AGC↑T-…5' Reaction Buffer 10X SpeedyOne Buffer Recommended Reaction Temperature 65°C Unit Definition At 65°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes. Methylation Effects Dam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect;CpG: Methylation does not affect. Thermal Inactivation Do not heat inactivate, use phenol chloroform extraction or column purification. Component 200 ul enzyme, 10X SpeedyOne buffer Storage -20°C Documents Manual MSDS
