STEMCELL Technologies 27215 Reversible Strainer (37 µm, Small, Sterile) The STEMCELL Technologies 27215 is a precision-engineered filtration tool designed for the preparation of high-purity single-cell suspensions. Featuring a 37 µm nylon mesh, this "small" format strainer is optimized for processing lower-volume samples while ensuring the removal of cell aggregates and tissue debris. The unique reversible design provides exceptional versatility: it can be used in the forward direction to filter suspensions into a collection tube, or inverted to recover concentrated cells that have been retained on the mesh. Individually sealed and certified sterile, the 27215 is a critical component for workflows in immunology, hematology, and stem cell research, particularly when preparing samples for magnetic cell separation (EasySep™) or flow cytometry. Technical Performance and Design 37 µm Precision Nylon Mesh: Provides a fine filtration barrier that is ideal for the isolation of smaller cell types, such as lymphocytes and hematopoietic stem cells, ensuring the removal of even small clumps that could interfere with downstream analysis. Reversible Functionality: The specialized frame allows for bi-directional flow. Filter "down" to remove debris, or filter "up" to collect and wash specific cell populations trapped on the mesh surface. Small-Volume Optimization: Specifically sized to fit standard 5 ml (12 x 75 mm) tubes and smaller vessels, minimizing dead volume and maximizing the recovery of precious or limited cell samples. Integrated Handle: Features an ergonomic tab that allows for secure handling with forceps or gloved hands, maintaining aseptic technique during transfer between tubes or buffers. Product Specifications Catalog Number: 27215 Pore Size: 37 µm Mesh Material: Nylon Format: Small Reversible Strainer Compatibility: Fits 12 x 75 mm (5 ml) polystyrene or polypropylene tubes. Sterility: Sterile (Gamma Irradiated); Individually Wrapped. Packaging: Available as individual units or in boxes of 50–100. Key Benefits & Applications EasySep™ Sample Prep: The recommended strainer for preparing single-cell suspensions from primary tissues or buffy coats prior to immunomagnetic cell isolation. Pre-FACS Filtration: Essential for removing micro-clumps before flow cytometry to prevent high-pressure fluidic clogs and ensure clean, reproducible gating. Primary Tissue Dissociation: Perfect for straining dissociated spleen, lymph node, or bone marrow samples where high cell viability and recovery are paramount. Cell Concentration: Allows researchers to "trap" cells on the mesh, perform a wash step, and then reverse the strainer into a fresh tube with a small volume of buffer to achieve a highly concentrated sample. All product names, logos, and brands are property of their respective owners.
