Description T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by using T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA. This kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. Figure 1: In vitro RNA transcription process Feature Up to 180 μg of RNA per reaction from 1 μg of the control template Optimized reaction system for the IVT process Decrease the dsRNA production Higher RNA integrity and purity Application In vitro RNA synthesis Components Components No. Name 10623ES50 (50 T) 10623ES60 (100 T) 10623ES70 (500 T) 10623-A T7 RNA Polymerase Mix 100 μL 200 μL 1 mL 10623-B 10×Transcription Buffer 100 μL 200 μL 1 mL 10623-C ATP (100mM) 100 μL 200 μL 1 mL 10623-D CTP (100mM) 100 μL 200 μL 1 mL 10623-E GTP (100mM) 100 μL 200 μL 1 mL 10623-F UTP (100mM) 100 μL 200 μL 1 mL 10623-G Control DNA Template (500ng/μL) 10 μL 20 μL 100 μL Shipping and Storage Dry ice transportation. Store at -15℃ ~ -25℃, valid for two years. Documents: Manuals 10623_MANUAL_HB220514_EN_1652931562792.PDF
