Description This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA. Note: G* is the first base of the RNA transcript. This product is produced in accordance with GMP regulations and provided in liquid form. Feature Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template Higher yield and easy to operate Lower endotoxins Tested for the absence of endonucleases, exonucleases, RNases Application Radiolabeled RNA probe preparation RNA generation for studies of RNA structure, processing and catalysis Expression control via anti-sense RNA mRNA, sgRNA synthesis Specification Source Recombinant E. coli with T7 RNA Polymerase gene Optimum Temperature 37℃ Storage Buffer 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v/v) glycerin，pH7.9 at 25℃ Unit Definition The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit. Components Components No. Name 10624ES90 (5,000 U) 10624ES97 (50,000 U) 10624 T7 RNA Polymerase GMP-grade (50 U/μL) 100 μL 1 mL Shipping and Storage T7 RNA Polymerase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year. Documents: Manuals 10623_MANUAL_HB220514_EN_1652931562792.PDF
