TFF1 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS91331-50, BS91331-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P04155(Human) Host: Rabbit Reactivity: Human Applications: WB, ICC/IF, IHC, IP, FC All Applications: WB:1:500 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 Background: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. pS2, also designated Md2, BCEI, TFF1 and pNR-2, is a trefoil protein that is highly expressed in normal gastric mucosa. pS2 has also been detected in a number of carcinomas including cancers of the breast, pancreas and stomach. pS2 is not present in normal breast tissue but is inducible by estrogen in MCF7 cells. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. The gene encoding pS2 maps to human chromosome 21q22.3. Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: TFF1 polyclonal antibody detects endogenous levels of TFF1 protein. Immunogen: Recombinant protein Conjugate: Unconjugated Modification: Unmodified Note: For research use only, not for use in diagnostic procedure. Extra Notes: ICC staining Estrogen Inducible Protein pS2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Estrogen Inducible Protein pS2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
