Description ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking. When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran. When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels", which may be downloaded below. Application 3D cell cultures Bioprinting Quantity Without peptide modification: Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink). Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link). The hydrogel volume will be reduced if peptide modifications are added. Components Material Quantity Concentration of reactive groups N-Dextran 2 x 600 µl 30 mmol/L MSDS 10x CB Buffer (pH 7.2) Phenol Red-free 2 x 600 µl n/a LAP, 10 mM 2 x 600 µl 10 mmol/L MSDS Water 3 x 1500 µl n/a MSDS Resources Product Datasheet (PDS): ToLuminate N-Dextran Protocol: Preparation of ToLuminate Photogels Protocol: Dissolution of Dextran-Based Hydrogels with 3-D Life Dextranase (Recovery of Cells) Related Products ToLuminate Photogels 3-D Life Hydrogels Ilex Life Sciences LLC is an authorized distributor of Cellendes GmbH.
