Product Specifications: Item# 60120: Recombinant Toxoplasma Sag1, Mic2,3 Antigen(Baculo) Concentration: See vial Mass/vial: 1mg Volume/vial: 500ul Diluent: 20 mM Na-Ac,20mM NaCl,pH 7+- Purity: >98% Stabilizer: None Preservative: None Storage: long term -75°C, Short term-20C° Physical State: Frozen Liquid Stability: 2 years at -75°C Application: In-Vitro Diagnostics, Immunization Description: Recombinant Recombinant Toxoplasma Sag1, Mic2,3 Antigen protein produced in the Baculovirus Expression System. molecular Weight: 47kD. Purification: This protein is purified by salt fractionation and ion-affity chromatography to >95% purity as determined by SDS-PAGE, reduced Specificity: This protein binds to Toxoplasma converted human serum polyclonal antibodies (IgG,IgM) in ELISA and Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests. Biological Activity: Not done Applications and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.
