Additional $40 shipping charge required for dry ice shipping Supplied in 2 x 50μl reactions per vial; 100μl per vial. 20 reaction size is delivered in 10 vials; 40 reaction size is delivered in 20 vials. Description Trans1-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 109 cfu/μg DNA (tested by pUC19 plasmid DNA). Genotype F-φ80(lacZ)ΔM15ΔlacX74hsdR(rk-, mk+)ΔrecA1398endA1tonA Features High transformation efficiency: >109 cfu/μg (pUC19 DNA). Fast-growing, colonies are visible in 8~9 hours. Resistance to T1 and T5 phage. Blue/white selection. Storage Competent Cell at -70°C for six months Notes Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure. References: Literature Journal IF Author Date Link Removal of antibiotic resistance genes from wastewater treatment plant effluent by coagulation water research 6.9419 Na Li,et al. 2017Mar The original link Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway Metabolic Engineering 6.859 Meng DC, et al. MOE Key Lab of Bioinformatics, Dep 2012 Jul The original link Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification analytical chemistry 6.32 Yi Hu,et al. 2016Nov The original link Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years journal of hazardous materials 6.065 Shuang Peng,et al. 2017Qct The original link Effect of fumigation with chloropicrin on soil bacterial communities and genes encoding key enzymes involved in nitrogen cycling Environmental Pollution 5.0989 Jun Li,et al. 2017Aug The original link
