Supplied in 2 x 50μl reactions per vial; 100μl per vial. 20 reaction size is delivered in 10 vials; 40 reaction size is delivered in 20 vials. Description Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). Genotype F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk-, mk+) supE44λ- thi-1 gyrA96relA1 phoA Features High transformation efficiency: >108 cfu/μg ( pUC19 DNA). Reduced recombination of cloned DNA. Blue/white selection. Storage Competent Cell at -70°C for six months Notes Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure. References: Literature Journal IF Author Date Link Fungicides induced triazole-resistance in Aspergillus fumigatus associated with mutations of TR46/Y121F/T289A and its appearance in agricultural fields Journal of Hazardous Materials 6.065 Jingbei Ren,et al. 2017Mar The original link Automatic Enumeration of Gold Nanomaterials at the Single-Particle Level Anal Chem 5.886 Xiao Xu, et al. 2015 Feb The original link Utilization of biodiesel by-product as substrate for high-production of ??-farnesene via relatively balanced mevalonate pathway in Escherichia coli Bioresource Technology 5.65 Shengping You et al 2017 Jun The original link The antitumor activity and preliminary modeling on the potential mechanism of action of human peroxiredoxin-5 Oncotarget 5.1657 Juanjuan Liu,et al. 2017Mar The original link Monitoring and evaluation of antibiotic resistance genes in four municipal wastewater treatment plants in Harbin, Northeast China Environ Pollut 5.0989 Wen Q, et al. 2016 Jan The original link
