TRA-zol Reagent is an improved version of the popular single-step total RNA isolation reagent developed by Chomczynski and Sacchi (1987). The RNA isolation (extraction) method based on this single reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin. TRA-zol Reagent performs well with easily scalable small or large amounts of tissue or cells, and many samples can be simultaneously extracted. This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein upon homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One milliliter of TRAzol is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 million cells, or 10 cm² of culture dish surface for cells grown in monolayer. This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein. The RNA extraction reagent has been widely used for isolating RNA, similar to the leading RNA Isolation Reagents. Resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR. Features and Benefits: • Easily scalable RNA isolation • Works with many sources: human, plant, yeast, bacterial, or viral • Better yields than traditional guanidine thiocyanate/cesium chloride methods • Cost-effective compared to other similar single-reagent RNA isolation reagents Reference Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem. 162: 156-159. 1987.
