Tris-Glycine Native Buffer (10X Transfer Buffer) Catalog number: B2010138 Lot number: Batch Dependent Expiration Date: Batch dependent Volume/Weight: 100 mL pH: 7.4 Supplied as: 10X concentrate (dilute to 1X with di-water before use) Appearance: Clear solution Applications: optimized buffer for use a transfer buffer during western blot applications. This buffer is filter sterilized. Storage: Room temperature Keywords: Transfer Buffer, Western Blot Transfer Buffer Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. References Taylor SC, Posch A. The design of a quantitative western blot experiment. Biomed Res Int. 2014;2014:361590. Jeong DE, Lee Y, Lee SV. Western Blot Analysis of C. elegans Proteins. Methods Mol Biol. 2018;1742:213-225. Burgess KA, Miller AF, Oceandy D, Saiani A. Western blot analysis of cells encapsulated in self-assembling peptide hydrogels. Biotechniques. 2017 Dec 1;63(6):253-260. Gallo-Oller G, Ordoñez R, Dotor J. A new background subtraction method for Western blot densitometry band quantification through image analysis software. J Immunol Methods. 2018 Jun;457:1-5. Swets M, Wouters A, Krijgsman D, van Vlierberghe RLP, Boot A, van Eendenburg JD, van Wezel T, Gelderblom H, van de Velde CJH, van den Elsen PJ, Kuppen PJK. HLA-G protein expression in colorectal cancer evaluated by immunohistochemistry and western blot analysis: Its expression characteristics remain enigmatic. Clin Immunol. 2018 Sep;194:80-86. 6: Bass JJ, Wilkinson DJ, Rankin D, Phillips BE, Szewczyk NJ, Smith K, Atherton PJ. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017 Jan;27(1):4-25. .
