Description In microbiological culture preparation, inconsistent nitrogen sources often lead to unstable bacterial growth, poor batch-to-batch reproducibility, and unpredictable fermentation performance. Researchers working with LB medium, diagnostic culture systems, or industrial fermentation frequently encounter variability caused by low-quality enzymatic digests or poorly controlled peptone raw materials. Tryptone Powder (TG217) is a bacteriological-grade enzymatic digest of casein designed to provide a consistent and biologically available source of peptides and amino nitrogen for microbial growth. It is widely used in microbiology, fermentation, recombinant protein expression, and diagnostic media formulation where reproducibility and nutrient consistency are critical. This product delivers a controlled profile of amino acids, peptides, and nitrogen compounds that support robust microbial proliferation in both routine laboratory workflows and scalable bioprocess applications. It is commonly used as a primary nitrogen source in LB broth and other complex culture media formulations requiring high growth performance and low variability between batches. Manufactured under controlled processing conditions and tested for microbiological quality, TG217 Tryptone Powder is optimized for laboratory environments requiring reliable performance, low contamination risk, and consistent nutrient composition across production-scale and research-scale applications. Specification Chemical & Physical Properties Appearance Fine, homogenous, light to medium tan yellow powder Amino Nitrogen (AN) ≥ 3.9% Total Nitrogen (TN) ≥ 10% Loss on Drying ≤ 6.0% Ash Content ≤ 15% pH (2% solution) 6.5 – 7.5 Microbiological Quality Standard Plate Count ≤ 5000 CFU/g Molds & Yeast ≤ 100 CFU/g Coliforms Negative Salmonella Negative Functional Performance Peptone Agar (USP) Satisfactory growth performance Storage Condition Room Temperature (dry and sealed) Features Consistent enzymatic casein hydrolysate for reproducible microbial culture performance High total nitrogen content (≥10%) supporting robust bacterial growth Balanced amino nitrogen profile (≥3.9%) for stable metabolic activity Low bioburden specification suitable for contamination-sensitive workflows Validated performance in USP peptone agar systems Optimized solubility for rapid media preparation and batch consistency Suitable for both research-scale and industrial fermentation systems Manufactured under controlled thermal processing conditions for stability Applications LB broth and complex microbial culture media preparation Escherichia coli cultivation and recombinant protein expression systems Industrial fermentation and microbial bioprocess development Diagnostic microbiology media formulation Enzyme production and metabolite biosynthesis workflows Pharmaceutical and veterinary microbiology research General bacterial growth media optimization studies Documents COA SDS Frequently Asked Questions What is tryptone powder used for in microbiology? Tryptone powder is used as a nitrogen-rich enzymatic digest of casein in microbial culture media. It supports bacterial growth by providing peptides and amino acids required for metabolism, commonly in LB broth and fermentation media. Is tryptone the same as peptone? Tryptone is a specific type of peptone produced by enzymatic digestion of casein, while peptone is a broader category that may come from different protein sources such as meat, soy, or casein. Can tryptone be used in LB medium preparation? Yes. Tryptone is one of the key components in LB (Luria-Bertani) medium, providing essential nitrogen and peptides that support E. coli and other bacterial growth. What is the difference between tryptone and casein hydrolysate? Tryptone is a refined enzymatic hydrolysate of casein with controlled peptide composition, while general casein hydrolysates may have less consistent peptide distribution and performance. Why is nitrogen content important in tryptone? Nitrogen content determines the availability of amino acids and peptides for microbial metabolism. Higher and stable nitrogen levels improve reproducibility in fermentation and culture media performance. Pexidartinib, PLX-3397, CSF1R inhibitor, c-Kit inhibitor, kinase inhibitor, tumor microenvironment, macrophage research, ATP-competitive inhibitor, oncology research compound For laboratory research or industrial use only. { "@context": "https://schema.org", "@type": "Product", "name": "Tryptone Powder (TG217)", "description": "Bacteriological grade enzymatic digest of casein used in microbial culture media, fermentation, and biotechnology applications.", "sku": "TG217", "brand": { "@type": "Brand", "name": "Bio Basic Inc." }, "additionalProperty": [ { "@type": "PropertyValue", "name": "Total Nitrogen", "value": "≥10%" }, { "@type": "PropertyValue", "name": "Amino Nitrogen", "value": "≥3.9%" } ], "category": "Microbiological Culture Media Components" } { "@context": "https://schema.org", "@type": "FAQPage", "mainEntity": [ { "@type": "Question", "name": "What is tryptone powder used for in microbiology?", "acceptedAnswer": { "@type": "Answer", "text": "It is used as a nitrogen-rich enzymatic digest of casein in microbial culture media such as LB broth to support bacterial growth." } }, { "@type": "Question", "name": "Is tryptone the same as peptone?", "acceptedAnswer": { "@type": "Answer", "text": "Tryptone is a specific type of peptone derived from casein, while peptone is a broader category from various protein sources." } } ] }
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