Varian / Agilent Bond Elut SPEC C18AR Solid Phase Extraction (SPE) Cartridges – 15 mg Sorbent Mass, 3 mL Column Volume, Monolithic Glass Fiber Disk Technology, 100/Pack (MPN: A5321920) The Varian A5321920 (now cataloged under Agilent Technologies as Bond Elut SPEC C18AR, MPN: A5321920) is a high-performance, 3 mL Solid Phase Extraction (SPE) column designed for rapid, reproducible sample cleanup and analyte enrichment in bioanalytical, pharmaceutical, and clinical LC-MS/MS workflows. Engineered with SPEC micro-disk technology, this column features a 15 mg octadecylsilane (C18AR / Acid Resistant) sorbent bed embedded within a uniform monolithic glass fiber disk. Unlike traditional loose-packed silica SPE cartridges, the SPEC disk eliminates particle channeling, prevents silica fines from entering the eluate, and drastically reduces void volume. The result is ultra-low elution volume requirements, fast flow rates under vacuum or pressure, and exceptional analyte recovery from complex biological matrices like plasma, serum, and urine. Technical Performance and Monolithic Disk Engineering Monolithic Glass Fiber Micro-Disk Design: Infuses the octadecylsilane (C18) stationary phase into a porous glass fiber matrix. This structure prevents bed disruption, particle channeling, and drying issues, delivering consistent flow rates and uniform extraction kinetics. Acid-Resistant C18AR Surface Chemistry: Specially modified hydrophobic C18 bonded phase designed to retain non-polar organic molecules, metabolites, and drug candidates while withstanding acidic pre-treatment protocols without phase stripping. Ultra-Low Elution Volume Capability: The low void volume of the 15 mg SPEC disk allows analytes to be eluted in micro-volumes (as low as 100 to 200 microliters), enabling direct LC-MS/MS injection or significantly shortening blow-down evaporation cycles. Elimination of Fines & Column Plugging: Because the sorbent matrix is bound within the fiberglass disk, no particulate fines wash out into final extracts, protecting analytical HPLC/UHPLC columns and mass spec sources from clogging. Product Specifications Part Number / MPN: A5321920 (Cross-listed as Varian A5321920 / Agilent Bond Elut SPEC A5321920) Manufacturer / Brand: Varian, Inc. / Agilent Technologies Sorbent Chemistry: SPEC C18AR (Acid-Resistant Octadecylsilane, Reversed-Phase) Sorbent Mass: 15 mg per cartridge Column Volume: 3 mL standard barrel Housing Material: Ultra-clean, medical-grade Polypropylene (PP) Packaging Quantity: Box of 100 individual cartridges Manifold Interoperability: Fits standard SPE vacuum manifolds, positive pressure processors (such as Agilent PPM-48), and automated SPE liquid handlers Key Benefits and Applications High-Sensitivity Bioanalysis & Pharmacokinetics: Essential for extracting low-concentration pharmaceuticals, metabolites, and biomarkers from whole blood, serum, plasma, and tissue homogenates. Toxicology & Clinical Screening: Provides fast, clean extraction of drugs of abuse, therapeutic agents, and steroid panels prior to quantitative LC-MS/MS or GC-MS runs. Streamlined Solvent Usage: Decreases hazardous organic solvent consumption during wash and elution steps, lowering operational costs and waste disposal overhead. All product names, logos, and brands are property of their respective owners.
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