VE-BEVS Complete Kit 30012 contains Product 10010 Insect Cell Line (VE-CL-01) and Product 20020 Transfer Vector (pAcVE.02). • VE-BEVS Insect Cell Line 10010 (1x10⁷ cells; frozen) (VE-CL-01) Enhanced expression of highly stable intracellular and secreted proteins. Harmonized Tariff: 3002.90.5150 • VE-BEVS Transfer Vector 20020 (10 µg) (pAcVE.02) Co-expression of 𝘷𝘢𝘯𝘬𝘺𝘳𝘪𝘯 gene for increased protein production; HBM signal sequence for direct secretion and N-terminal 8xHis-tag for ease of purification of protein of interest. Vankyrin-Enhanced (VE) baculovirus transfer vector designed for high levels of recombinant gene expression under the control of the Autographa californica nucleopolyhedro virus (AcMNPV) polyhedrin promoter. Advantages VE-BEVS Insect Cell Line 10010 Engineered monoclonal Sf9 cell line stably expresses a vankyrin gene Mycoplasma free Up to 8-fold increase in protein yield as compared to parental Sf9 cells Prolonged longevity of cells after infection with a baculovirus compared to parental Sf9 cells Increased baculovirus titer Compatible with all conventional BEVS Reduced cost and labor Expression of recombinant protein may need to be optimized by testing different time points and MOIs. Transfer Vector 20020 (pAcVE.02) This baculovirus transfer vector integrates the co-expression of a vankyrin gene from the p10 promoter for the increased production of gene of interest from the polyhedrin promoter with: Honey bee melittin signal sequence for direct secretion and N-terminal 8x his-tag for ease of purification Documents VE-BEVS Cell Line Instructions [PDF] Transfer Vector pAcVE.02 [PDF] pAcVE.02 Instructions [PDF] VE-BEVS Cell Line FAQs [PDF] VE-BEVS Licensing Contract service for introducing the vankyrin gene into a custom insect cell line is available upon request. Patent information:VE-BEVS Cell Line United States Patent 7,842,493 [PDF]VE-BEVS Transfer Vector United States Patent 7,629,160 [PDF]
