VoluScan™ is an epifluorescence mesoscope that enables 1- or 2-color functional imaging of large sample volumes at a cellular resolution. VoluScan™ is designed to image fluorescent sensors (like GCaMP, jRGECO, dLight, etc.) in large 3D biological samples such as organoids, assembloids, brain slices, and cell cultures, but can also be used to study cell migration, division, or other processes. Furthermore, the system is compatible with Tokai Hit incubation chamber and can synchronize imaging with other systems using external digital triggers. Thus, VoluScan™ pairs readily with Light Sources (for optogenetics or uncaging), electrophysiology recordings, liquid infusion, etc. Gen.2 of VoluScan is fitted with a motorized 3D stage to sequentially image multiple regions of large sample plates (well culture plates, Petri dishes, …) for high-throughput imaging. Simply leaving VoluScan overnight after defining regions of interest can greatly accelerates data collection.