Yeast Nitrogen Base w/o Amino Acids (S507)
Yeast Nitrogen Base without Amino Acids | YNB Powder Yeast Nitrogen Base without Amino Acids is a dehydrated culture medium for yeast classification, amino acid requirement testing , and carbohydrate utilization studies. Description In yeast genetics, nutritional requirement testing, and carbohydrate utilization studies, researchers often need a defined yeast medium that supports controlled supplementation rather than a fully complete growth formula. When amino acids or carbon sources are already present in the base medium, it becomes difficult to determine whether yeast growth is driven by the organism's true nutritional capability or by unintended background nutrients. Yeast Nitrogen Base without Amino Acids is a dehydrated culture medium designed for the cultivation and classification of yeasts based on amino acid and carbohydrate requirements. This YNB without amino acids formulation contains essential vitamins, inorganic salts, trace elements, and ammonium sulfate as a nitrogen source, while leaving out selected amino acids and a carbohydrate source so users can supplement the medium according to their experimental design. The medium is commonly used as a defined yeast minimal medium for yeast classification, auxotrophic strain selection, yeast nutritional requirement testing, carbohydrate assimilation studies, and molecular genetics applications involving Saccharomyces, Candida, and related yeast species. Dextrose or another carbohydrate source, along with the desired amino acid supplement, should be added before use. Specification Product Name Yeast Nitrogen Base without Amino Acids Synonyms YNB without amino acids; YNB w/o amino acids; yeast nitrogen base powder Product Type Dehydrated culture medium Application Yeast cultivation, yeast classification, amino acid requirement testing, carbohydrate requirement testing Final pH 5.4 ± 0.2 at 25.0°C Appearance Homogeneous, free-flowing, off-white powder Prepared Medium Appearance Clear and almost colorless Storage Store sealed dehydrated medium at 2-30°C. Protect from moisture and light. Use Limitation For laboratory research use only. Not for human or animal drug use. Formula per Liter Ammonium Sulfate 5.0 g Monopotassium Phosphate 1.0 g Magnesium Sulfate 0.5 g Sodium Chloride 0.1 g Calcium Chloride 0.1 g Inositol 2 mg Niacin 0.4 mg Pyridoxine Hydrochloride 0.4 mg Thiamine Hydrochloride 0.4 mg Calcium Pantothenate 0.4 mg Manganese Sulfate 0.4 mg Zinc Sulfate 0.4 mg Ferric Chloride 0.2 mg Sodium Molybdate 0.2 mg p-Aminobenzoic Acid 0.2 mg Riboflavin 0.2 mg Boric Acid 0.5 mg Potassium Iodide 0.1 mg Copper Sulfate 0.04 mg Biotin 0.002 mg Folic Acid 0.002 mg Grams per liter may be adjusted or the formula supplemented to obtain desired performance. Features Defined yeast medium: Provides controlled nutrients for yeast cultivation and nutritional requirement testing. Without amino acids: Designed for amino acid requirement testing and auxotrophic yeast selection workflows. Carbohydrate-free base: Allows researchers to add dextrose or an alternative carbon source for carbohydrate utilization studies. Contains ammonium sulfate: Supplies inorganic nitrogen while enabling targeted supplementation of amino acids and carbohydrates. Vitamin and salt system: Includes essential vitamins, inorganic salts, and trace elements required for yeast growth. Suitable for molecular genetics: Useful in yeast genetics, yeast classification, and defined medium preparation. Applications Yeast classification: Used for classifying yeasts based on amino acid and carbohydrate requirements. Amino acid requirement testing: Supports controlled studies where selected amino acids are added to evaluate yeast nutritional needs. Carbohydrate requirement testing: Allows addition of dextrose or other carbohydrate sources for carbon source utilization studies. Yeast genetics: Suitable for molecular genetics applications involving defined yeast media and selective growth conditions. Auxotrophic yeast selection: Useful for culturing and screening yeast strains with defined nutritional supplementation. Yeast minimal medium preparation: Provides a base for preparing synthetic yeast medium with user-defined supplements. Saccharomyces cultivation: Supports growth studies involving Saccharomyces species when supplemented with required additives. Candida and related yeast research: Suitable for yeast cultivation and cultural response testing with appropriate supplements. Microbiology education and QC workflows: Can be used in controlled demonstrations of yeast growth requirements and medium supplementation. Preparation Prepare a 10X solution by dissolving 6.7 g of Yeast Nitrogen Base without Amino Acids, 5 g of dextrose or an equivalent amount of another carbohydrate, and 5-10 mg of the desired amino acid in 100 mL of distilled or deionized water. Warm slightly to dissolve and mix well. Do not bring to a boil. Do not autoclave. Filter-sterilize the solution and store the 10X stock solution at 2-8°C. To prepare the final medium, aseptically pipette 0.5 mL of the 10X solution into 4.5 mL of sterile distilled water. Mix thoroughly by shaking before inoculation. Quality Control Specifications The powder is homogeneous, free flowing, and off-white. The prepared medium is visually clear and almost colorless. Expected cultural response is observed after 2-5 days at 25.0°C. Organism Result with Additives Candida albicans ATCC 10231 Good Growth Kloeckera apiculata ATCC 9774 Good Growth Saccharomyces pastorianus ATCC 9080 Good Growth Storage Store the sealed bottle containing the dehydrated medium at 2-30°C. Once opened and recapped, place the container in a low-humidity environment at the same storage temperature. Protect from moisture and light. Discard the dehydrated medium if it is no longer free flowing or if the color has changed from the original off-white appearance. Documents INFO SDS Frequently Asked Questions What is Yeast Nitrogen Base without Amino Acids used for? Yeast Nitrogen Base without Amino Acids is used as a defined dehydrated culture medium for yeast classification, yeast cultivation, amino acid requirement testing, carbohydrate requirement testing, and yeast molecular genetics applications. Does this YNB medium contain a carbon source? No. This medium does not contain a carbohydrate source. Dextrose or another selected carbon source should be added according to the intended yeast growth or carbohydrate utilization study. Does Yeast Nitrogen Base without Amino Acids contain ammonium sulfate? Yes. This formulation contains ammonium sulfate as an inorganic nitrogen source. It should not be confused with YNB formulations labeled without amino acids and without ammonium sulfate. Can this medium be used for auxotrophic yeast selection? Yes. Because it is supplied without amino acids and without a carbohydrate source, it can be supplemented with defined amino acids and carbon sources for auxotrophic yeast selection and nutritional requirement studies. How should a 10X YNB stock solution be prepared? Prepare a 10X solution by dissolving 6.7 g of YNB without amino acids, 5 g of dextrose or another carbohydrate, and 5-10 mg of the desired amino acid in 100 mL distilled or deionized water. Warm slightly if needed, mix well, and filter-sterilize. Can Yeast Nitrogen Base without Amino Acids be autoclaved? No. The solution should not be boiled or autoclaved. Filter sterilization is recommended because vitamins and other heat-sensitive components may be affected by high-temperature sterilization. What organisms are used for quality control testing? Quality control cultural response is evaluated using organisms such as Candida albicans ATCC 10231, Kloeckera apiculata ATCC 9774, and Saccharomyces pastorianus ATCC 9080 with appropriate additives. How should the dehydrated medium be stored? Store the sealed dehydrated medium at 2-30°C. After opening, recap tightly and keep in a low-humidity environment protected from moisture and light. Yeast Nitrogen Base without Amino Acids powder for yeast classification and amino acid requirement testing YNB without amino acids dehydrated culture medium for yeast genetics and carbohydrate utilization studies Yeast nitrogen base powder for Saccharomyces Candida and Kloeckera cultivation with defined supplements Yeast Nitrogen Base without Amino Acids, YNB without amino acids, YNB w/o amino acids, yeast nitrogen base powder, dehydrated culture medium, yeast classification medium, defined yeast medium, yeast minimal medium, microbiological media, yeast cultivation, amino acid requirement testing, carbohydrate requirement testing, auxotrophic yeast selection, yeast genetics, molecular genetics applications, Wickerham formula, ammonium sulfate, dextrose supplementation, filter sterilize, 10X stock solution, Saccharomyces, Candida albicans, Kloeckera apiculata, Saccharomyces pastorianus For laboratory research or industrial use only.
