DNase I is an endonuclease that can digest single- or double-stranded DNA. It can hydrolyze phosphodiester bonds to produce mono- and oligodeoxynucleotides containing a 5'-phosphate group and a 3'-OH group.The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, Zn2+, etc. In the presence of Mg2+, DNase I can randomly cleave any site of double-stranded DNA; while in the presence of Mn2+, DNase I can cleave DNA double-stranded at the same site, forming blunt ends or sticky ends with 1-2 nucleotides protruding. It can be used for the processing of various RNA samples. Features Recombinant source. RNase-free. High enzymatic cleavage efficiency. More suitable for applications sensitive to RNase. Applications Removal of gDNA before RNA extraction or reverse transcription. Removal of template DNA in in vitro transcription. rRNA removal in RNA library construction and sequencing. Nick translation for DNA labeling. DNase I footprinting assay experiment. Specifications Expression Host Recombinant E. coli with Dnase I gene Unit Definition The amount of enzyme required to increase the absorbance at 260 nm of the reaction solution by 0.001in1minute at 25℃ and pH 5.0 using calf thymus DNA as the substrate is defined as one activity unit(Kunitz Unit) Components Components No. Name 10325ES80 10325ES91 10325-A Recombinant DNase I (RNase-free) -2 U/μL 500 μL 2.5 mL 10325-B DNase I Reaction Buffer (10×) 1 mL 5 mL Figures No RNase Contamination DNase I was incubated with RNA substrate, and changes in the RNA bands were analyzed by agarose gel electrophoresis. The results showed that Yeasen DNase I contains no detectable RNase contamination. Figure 1. RNase contamination assay C: Control Efficient DNA Removal Without Affecting RNA Products DNase I from Yeasen and two supplier brands, T* and N*, was used to remove DNA templates from in vitro transcription (IVT) reactions. The results showed that Yeasen DNase I achieved DNA template removal efficiency comparable to that of leading supplier brands. Figure 2. In vitro transcription - Template DNA removal: C: No DNase I control; T: supplier brand T; N: supplier brand N; M: Marker. NGS Library Preparation Yield Comparable to Imported Brands DNase I from Yeasen and supplier brand T* was used during rRNA depletion in RNA library preparation. The results showed that RNA library yields obtained using Yeasen DNase I were comparable to those obtained using the supplier brand. Figure 3. Application of DNase I in RNA library preparation T*: supplier brand T*
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