Ribonuclease A (RNase A) is a single-stranded polypeptide containing 4 disulfide bonds with a molecular weight of about 13.7 kDa. RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. Specifically, the cleavage recognizes the phosphodiester bond formed by the 5'-ribose of a nucleotide and the phosphate group on the 3'-ribose of the adjacent pyrimidine nucleotide, so that the 2', 3' - Cyclic phosphates are hydrolyzed to the corresponding 3'-nucleoside phosphates (eg, pG-pG-pC-pA-pG is cleaved by RNase A to generate pG-pG-pCp and A-PG).RNase A is the most active in cleaving single-stranded RNA .Recommended working concentration is 1-100 μ G/mL, compatible with various reaction systems. Low salt concentration (0-100 mM NaCl) can be used to cut single-stranded RNA, double-stranded RNA, and RNA chains formed by RNA-DNA hybridization. However, at high salt concentration (≥ 0.3 M), RNase A only specifically cleaves single-stranded RNA.RNase A is most commonly used to remove RNA during the preparation of plasmid DNA or genomic DNA. Whether or not DNase is active during the preparation process can easily affect the reaction. The traditional method of boiling in a water bath can be used to inactivate DNase activity. This product does not contain DNase and protease, and does not require heat treatment before use. In addition, this product can also be used in molecular biology experiments such as RNase protection analysis and RNA sequence analysis. Features Chromatographically purified Type II-A, >= 80 Kunitz units/mg protein Applications Removal of RNA during genomic DNA preparation RNA enzyme protection analysis RNA sequence analysis Specifications Appearance Powder Quantity 100 mg/1 g Product type RNase A Components Components No. Name 10407ES60 10407ES80 10407 Ribonuclease A (RNase A) 100 mg 1 g Storage The product should be stored at -25℃ ~ -15℃ for two years. Documents: Safety Data Sheet 10407_MSDS_HB250721 Manuals 10407_Manual_HB20260616 Related blog： What is the magic of RNase A? Uncovering its diverse applications
