S-Adenosyl methionine (S-Adenosyl methionine, SAM) is an auxiliary substrate involved in the methyl transfer reaction, which in the body is composed of adenosine triphosphate and methionine in the methionine adenosyltransferase under the action of synthesis. SAM is prepared in a solution of 10 mM HCl, 10% Ethanol, and pH 4 at 25℃. This product is produced by GMP process requirements and provided in liquid form. Features Validated, product-specific processes and analytical methods Product-specific stability Documentation follows applicable GMP guidelines AOF production process and raw materials (TSE & BSE) Nitrosamine statement Regulatory support documents available Large-scale production Applications Enzymatic capping process for mRNA vaccines and therapeutics Enzymatic methylations of biopolymers Specifications CAS No 485-80-3 Formula C15H23N6O5S+ Molecular Weight 399.44 g/moL Purity (HPLC) ＞ 90% Content 32 mM ± 2 mM Composition (1X) 10 mM HCl, 10% Ethanol pH 4 at 25℃ Structure Components Components No. Name 10619ES02 10619ES25 10619ES50 10619ES76 10619 S-adenosylmethionine (SAM) GMP-grade (32 mM) 0.5 mL 25 mL 50 mL 500 mL Shipping and Storage The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Figures Purity Assessment Figure 1. The purity (HPLC) of SAM product could be over 98% (Figure 1A) and the optical purity（ee, %）of (S, S) -SAM isoform always be about 70% (Figure 1B). Enzyme Residual Detection Figure 2. No enzyme residue was detected by agarose gel electrophoresis. A 20 µl reaction in the buffer containing 500 ng of Hind III digest of λDNA and 2 µl of S-adenosylmethionine (SAM) incubated for 4 hours at 37ºC results in no difference compared with the control ( SAM free in the reaction system）by agarose gel electrophoresis (Figure 2A). A 20 µl reaction in the buffer containing 500 ng of pUC19 plasmid and 2 µl of S-adenosylmethionine (SAM) incubated for 4 hours at 37ºC results in no difference compared with the control ( SAM free in the reaction system）by agarose gel electrophoresis (Figure 2B). Functional Test and Verification Figure 3 The capping efficiency of YEASEN post-transcriptional capping reaction could be close to 99%. A 20 µl reaction in the buffer containing 1 µg of λDNA, 1 unit of M. SssI (CpG Methyltransferase), and 160 µM S-adenosylmethionine (SAM) is incubated for 1 hour at 37°C. The resulting DNA is resistant to digestion with BstUI as determined by agarose gel electrophoresis (Figure 3A). 10 μg RNAs were denatured by incubation at 65°C for 5 min before capping. A 20 μL post-transcriptional capping reaction was set up and incubated at 37°C for 2 hours in a PCR machine. Transcripts were purified by magnetic beads (Cat#12602). Then the capping efficiency is detected by LC-MS (Figure 3B). FAQ Q: Are there any enzyme residues? A: No residual nucleic acid exonuclease, endonuclease, or Rnase. Documents: Safety Data Sheet 10619_MSDS_HB260528 Manuals 10619_Manual_HB20260302 Related blog： DNase I Applications
