N1-Me-Pseudo UTP sodium solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics, and changes the innate immune receptor and in vitro transcription. The interaction of RNA has a wide range of applications in the field of therapy and diagnosis.This product is produced in accordance with GMP process requirements and provided in liquid form. Feature Validated, product-specific process and analytical methods Product-specific stability Documentation follows applicable GMP guidelines AOF production process and raw materials (TSE & BSE) Nitrosamine statement Regulatory support documents available Large-scale production Nucleotide in the multiple salt form（Na+, Tris etc) always available to meet different downstream application needs Application Modified RNA synthesis and amplification Building block for in vitro transcription Specification CAS No 1428903-59-6 (free acid) Formula C10H14N2Na3O15P3 Molecular Weight 564.11 g/moL Purity (HPLC) ≥ 99% Content 100 mM ± 3 mM Structure Component Components No. Name 10651ES20/70 10651ES80 10651ES10 10651 N1-Me-Pseudo UTP sodium solution GMP-grade (100 mM) 20 μL/100 μL 1 mL 10 mL Figures Standard RNA Synthesis Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit. The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1. Capped RNA Synthesis Figure 2. Synthesis of capped RNA in vitro. The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.
Related
Related