Pseudouridine-5-triphosphate solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.This product is produced in accordance with GMP process requirements and provided in liquid form. Feature Validated, product-specific processes and analytical methods Product-specific stability Documentation follows applicable GMP guidelines AOF production process and raw materials (TSE & BSE) Nitrosamine statement Regulatory support documents available Large-scale production The increased IVT yield and the decreased dsRNA content under the optimized Tris NTP reaction system Application RNA synthesis and amplification Building block for in vitro transcription Specification CAS No. 1175-34-4 (free acid) Molecular formula C9H15N2O15P3 (free acid) Molecular weight 484.14 g/mol (free acid) Purity ≥ 99% Content 100 mM ± 3 mM Structure Component Components No. Name 10656ES20 10656ES70 10656ES80 10656ES10 10656 Pseudo UTP Tris Solution GMP-grade (100 mM) 20 μL 100 μL 1 mL 10 mL Storage The product should be stored at -25℃ ~ -15℃ for two years. Figures Increased the IVT yield Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. Decreased the dsRNA content Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by Dot Blot method. Documents Safety Data Sheet 10656_MADS_HB260718 Manuals 10656_Manual_HB230112
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