Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted. Detection principle as shown in the figure: Figure 1: Schematic diagram of the firefly luciferase detection principle YEASEN Biotech's Bright-One Step Luciferase Assay Kit features easy operation, high sensitivity, and strong signal intensity. It is suitable for experiments that require high sensitivity. There is no need to wash or collect cells before the experiment, and it can be used directly. The lysis and detection of cells are completed in one step, eliminating the need to mix reagents, saving experimental time. Features Long signal half-life: The signal half-life is 30 min, meeting the needs of high-throughput experiments. Strong fluorescence signal: The signal value can reach 8 orders of magnitude, with a large detection window. Convenient operation: One-step procedure, avoiding complicated experimental processes. Applications Signaling Pathway Research Candidate Drug Screening T Cell Killing Assay Specifications Form liquid Luciferase Firefly Signal Half-life 30 min Number of Product Components 1 Workflow One Step Components Components No. Name 11412ES60 11412ES80 11412ES81 11412 Bright -One Step Luciferase Assay Kit 10 mL 10×10 mL 100 mL Shipping and Storage Bright -One Step Luciferase Assay Kit products should be stored at -80℃ for 1 years. Figures Figure1. YEASEN Bright -One Step has better stability and higher signal values compared to its competitors. Documents: Safety Data Sheet 11412_MSDS_HB241231 Manuals 11412_Manual_Ver.EN20250903
