Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted. Detection principle as shown in the figure: Figure 1: Schematic diagram of the firefly luciferase detection principle YEASEN Biotech's Steady-One Step Luciferase Reporter Gene Detection Kit is characterized by its ease of operation and a long half-life of over 5 hours, with minimal luminescence value changes in the first 2 hours. It is particularly suitable for ultra-high throughput or 384-well plate detection. There is no need to wash or collect cells before the experiment; the reagent can be used directly, with cell lysis and detection completed in one step, eliminating the need for mixing reagents, thus saving experimental time. This kit achieves a relative balance between signal strength and stability, maintaining a relatively high signal strength while keeping the signal intensity relatively stable over a certain period, ensuring that the experimental results are accurate and reliable. The half-life typically reaches over 1 hour. The product has a wide linear range for sample determination. In a 96-well plate, it can exhibit a good linear relationship within a range of 200,000 cells. Features Long signal half-life: The signal half-life is 5 hour, meeting the needs of high-throughput experiments. Strong fluorescence signal: The signal value can reach 7 orders of magnitude, with a large detection window. Convenient operation: One-step procedure, avoiding complicated experimental processes. Applications Signaling Pathway Research Candidate Drug Screening T Cell Killing Assay Specifications Form liquid Luciferase Firefly Signal Half-life 5 h Number of Product Components 1 Workflow One Step Components Components No. Name 11413ES60 11413ES80 11413ES81 11413 Steady -One Step Luciferase Assay Kit 10 mL 10×10 mL 100 mL Shipping and Storage Steady -One Step Luciferase Assay Kit products should be stored at -80℃ for 1 years. Figures Figure1. YEASEN Steady -One Step has better stability and higher signal values compared to its competitors.. Documents: Safety Data Sheet 11413_MSDS_HB241231_EN.PDF Manuals 11413_Manual_HB241230_EN.pdf
