Hieff NGS™ OnePot EvoMax DNA Library Prep Kit(Premixed Version) _ 12112ES

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Hieff NGS™ OnePot Pro DNA Library Prep Kit V4 is is an advanced enzymatic DNA library preparation kit designed for compatibility with both Illumina™ and MGI™ next-generation sequen cing (NGS) platforms. By utilizing high-efficiency enzymatic fragmentation, this kit eliminates the need for traditional mechanical shearing methods such as sonication, streamlining the workflow and minimizing hands-on time. The kit integrates DNA fragmentation and end-repair into a single-step reaction, significantly reducing overall library preparation time and cost. It is suitable for a broad range of sample types, including plant genomes, animal genomes, microbial genomes, and more, with a flexible DNA input range from 1 ng to 1 μg. The optimized enzymatic fragmentation ensures consistent and uniform library fragment sizes across different species, minimizing variation and enhancing sequencing performance. The prepared libraries are fully compatible with Illumina™ or MGI™ adapters and primers, enabling seamless downstream sequencing on respective platforms. Features Ultra-Simplified Pre-Mix An all-in-one master mix streamlines library preparation with minimal pipetting and hands-on time. Broad Input Compatibility Supports 1 ng–1 μg DNA inputs, including gDNA, full-length cDNA, and other DNA samples. Low-Bias Fragmentation Delivers random DNA fragmentation with minimal sequence bias for accurate genome representation. Exceptional Library Performance High-efficiency library conversion and high-fidelity amplification generate high-complexity libraries with robust yield for reliable downstream sequencing. Stable Pre-Mixed Formula Optimized pre-mixed formulation delivers outstanding stability, reproducible performance, and reduced hands-on variability. Specifications Cat.No. 12112ES24 / 12112ES96 / 12112ES97 Size 24 T / 96 T / 96T(Plate format) Components Components No. Name 12112ES24 12112ES96 12112ES97(Plate format)* 12112-A Smearase™ Enzyme mix 480 μL 2×960 μL 8×240 μL 12112-B Ligation Ready Mix 1080 μL 3×1440 μL 16×270 μL 12112-C 2×Ultima HF Amplification Mix 2.0 600 μL 2×1200 μL 8×300 μL Layout of the Pre-packaged, plate-based library prep kit format. Storage This product should be stored at -25~-15℃ for one year.. Application DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing; Figures 1. Ultra-Simplified Pre-Mix Figure 1. The ultra-simplified 3-tube pre-mix workflow of the EvoMax DNA Library Prep Kit. 2. Broad Species Compatibility Figure 2. Library uniformity testing across different species using 12972 under identical fragmentation conditions. Different DNA samples were fragmented with 12972 under identical conditions. The libraries showed consistent sizes (~450 bp / ~320 bp) with good uniformity across species. 3. Strong Stability Figure 3. Stability assessment of the EvoMax DNA Library Prep Kit. The library performance remained comparable to the control after 2 weeks at 4°C and 8 months of real-time stability at -20°C. Documents: Safety Data Sheet 12112_MSDS_HB260629 Manuals 12112_Manual_HB20260629 Show more