Hieff NGS™ OnePot Evo DNA Library Prep Kit (Enzymatic, For FFPE, V4) _ 12193ES

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Hieff NGS™ OnePot Evo DNA Library Prep Kit is a next-generation enzymatic library preparation kit compatible with both Illumina and MGI high-throughput sequencing platforms. Compar ed to traditional library construction methods, this kit replaces time-consuming and instrument-dependent sonication with a high-quality, low-bias fragmentation enzyme. It uniquely integrates the fragmentation, end repair, and dA-tailing steps into a single reaction, significantly reducing hands-on time, cost, and workflow complexity. The ligation module features pre-mixed enzyme and buffer for streamlined operation, making the kit highly suitable for automation. Building upon the previous version, this updated formulation further reduces GC bias, enhances end-repair and dA-tailing efficiency, and improves reagent stability. It also incorporates a newly optimized ligase for superior adapter ligation performance. The kit is fully compatible with standard Illumina or MGI adapters and primers. Ideal for whole-genome sequencing of animals, plants, microbes, and challenging clinical samples. Features Wide input range: Compatible with 5 ng–1 µg of genomic DNA, full-length cDNA, or other double-stranded DNA High-quality fragmentation enzyme: Enables random dsDNA cleavage with minimal sequence bias, integrating fragmentation, end repair, and dA-tailing in a single step High-fidelity, high-efficiency PCR enzyme: Maximizes library yield and complexity while preserving sequence accuracy FFPE-compatible: Delivers robust performance with degraded or formalin-fixed DNA samples Rigorous quality control: Each production batch undergoes stringent performance validation and stability testing Specifications Cat.No. 12193ES08 / 12193ES24 / 12193ES96 Size 8 T / 24 T / 96 T Components Components No. Name 12193ES08 12193ES24 12193ES96 12193-A Smearase™ Buffer 40 μL 120 μL 480 μL 12193-B Smearase™ Enzyme 80 μL 240 μL 960 μL 12193-C Rapid Ligase Master Mix 280 μL 840 μL 4×840 μL 12193-D 2× Ultima HF Amplification Mix 200 μL 600 μL 3×800 μL [Note]: The kit components are compatible with both Illumina &MGI sequencing platform, if the complete adapter was used, Hieff NGS Primer Mix (Yeasen Cat#12190 or Cat#12191) in needed. Storage This product should be stored at -25~-15℃ for one year.. Application DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing; Figures 1. Workflow Figure 1. The workflow of OnePot Pro DNA Library Prep Kit 2. Flexible and High-Efficiency Fragmentation Figure 2. Fragment size distribution of 250 ng human gDNA using the 12193 fragmentation module. Calf gDNA (250 ng) was fragmented at 30°C ~35°C for 5 min, 10, 15, 20, 30. The distribution of recovered insert sizes is shown, demonstrating stable and uniform fragmentation across the 5–30 minute range. Different DNA samples were fragmented with 12194 under identical conditions. The libraries showed consistent sizes (~450 bp / ~320 bp) with good uniformity across species. Figure 3. Comparison of soft-clipping rates (%) and false chimeric read rates (%) across human gDNA, ctDNA, and FFPE DNA samples. The results show that 12194 exhibits lower or comparable soft-clipping rates (%) and false chimeric rates(%). 3. Strong Stability Figure 4. Library yield stability after storage at 4°C and 25°C for 4 weeks. No decrease in library yield was observed after 4 weeks of storage at 4°C and 25°C, highlighting the kit’s suitability for automation. Documents: Manual 12193_Manual_HB20260430 Show more