Hieff NGS™ MaxUp rRNA Depletion Kit (Plant) is designed to remove rRNA (including cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA, chloroplast 16S and 23S rRNA) from plant total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples. Feature Strong Specificity: Efficiently removes rRNA from various plant samples Broad Input Compatibility: Suitable for a wide range of input amounts (10 ng to 1 μg). Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results. Specifications Depletion Technology RNase H Sample Type Total RNA of Plant Final Product Type mRNA and other non coding RNAs No. of Reactions 24/96 Preps Starting Material Amount 100 ng~1 μg total RNA Target Remove rRNA Components Components No. Name 12254ES24 12254ES96 12254-A Hybridization Buffer 72 μL 288 μL 12254-B Probe Mix (Plant) 72 μL 288 μL 12254-C RNase H Buffer 72 μL 288 μL 12254-D RNase H 48 μL 192 μL 12254-E DNase I Buffer 660 μL 2×1320 μL 12254-F DNase I 60 μL 240 μL Applications rRNA Removal from Total RNA of Plant Roots, Seeds, and Leaves; mRNA and Non-coding RNA Analysis Storage This product should be stored at -25~-15℃ for 12 months. Figures 1. Efficient Removal of rRNA from Diverse Plant Species: This product has been verified for species including Arabidopsis, rice, wheat, cotton, corn, rapeseed, sunflower, tomato, walnut, strawberry, watermelon, barley, sweet potato, potato, mulberry, etc. Figure 1. Efficient rRNA Removal using MaxUp rRNA Depletion Kit (Plant) 1 μg of total RNA from various plants was used to construct RNA libraries with the YEASEN Dual-mode RNA Library Prep Kit (Cat#12308) and rRNA Depletion Kit (Cat#12254). The percentage of rRNA reads before and after depletion was calculated to evaluate the rRNA removal efficiency. 2.Wide Compatibility with Different Input Amounts Figure 2. Robust rRNA Removal Across Varying Input Amounts Library preparation was performed using 12254 and RNA Library Preparation Kit (Cat#12308, with strand-specific protocols) on various plant sample types(Input RNA: 10 ng to 1 μg). The rRNA residual rate remained consistently low. Documents: Safety Data Sheet 12254-COMPONENT A-MSDS-HB220428 12254-COMPONENT B-MSDS-HB220428 12254-COMPONENT C-MSDS-HB220428 12254-COMPONENT D-MSDS-HB220428 12254-COMPONENT E-MSDS-HB220428 12254-COMPONENT F-MSDS-HB220428 Manuals 12254_Manual_Ver.EN20250923.pdf
