Hieff NGS™ MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) 2.0 utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion. Features Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples. Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg). High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples. Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results. Components Components No. Name 12726ES12 12726ES24 12726ES96 12726-A Hybridization Buffer 36 μL 72 μL 288 μL 12726-B Human Probe Mix (rRNA & ITS/ETS) 24 μL 48 μL 192 μL 12726-C RNase H Buffer 36 μL 72 μL 288 μL 12726-D Thermostable RNase H 4 μL 48 μL 192 μL 2726-E DNase I Buffer 330 μL 660 μL 2x1320 μL 12726-F DNase l 30 μL 60 μL 240 μL Applications Gene Expression Research Alternative splicing analysis Detection and discovery of LncRNA(Non coding RNA) dentification of selective polyadenylation sites Fusion gene detection Storage This product should be stored at -25~-15℃ for 12 months. Figures 1. Broad Compatibility Across Input Amounts and Sample Types Figure 1. Superior rRNA Removal Efficiency Across a Broad Input Range Library preparation was performed using the MaxUp™ rRNA Depletion Kit and a strand-specific RNA Library Preparation Kit on human, mouse, and rat total RNA samples. Unlike traditional kits limited to 1 µg input, MaxUp™ demonstrates consistent and efficient rRNA removal across a wide range of input amounts (100 ng to 1 µg), achieving 98% depletion efficiency with a 100 ng input. This ensures high-quality, low-background library construction, making it highly compatible with low-quality samples (DV200 < 50%) for clinical and archival tissue research. 4. Automation-Ready Stability Figure 4. Enhanced Stability and Automation Compatibility MaxUp™ features a stabilized RNase H system that eliminates the need for fresh enzyme preparation, making it ideal for automated, high-throughput workflows. The kit maintains superior depletion efficiency (>95%) under various storage conditions, including extended periods at 37°C, RT, and 4°C. Documents: Safety Data Sheet 12726_MSDS_HB250805_EN.PDF Manuals 12726_Manual_HB20260401.pdf Related Blogs： rRNA Depletion Kit – Efficient rRNA Removal Across All Sample Types
