Hieff NGS™ FFPE DNA Repair Reagent is specifically designed for FFPE samples to repair varying degrees of damage in low-quality FFPE DNA, enhancing library yield and sequencing quality. This product can be directly used with Yeasen Hieff NGS™ Library Preparation Kits (Cat#12197/Cat# 12927) and the A-tailing module (Cat# 12608), with compatible systems that can repair 5 ng to 1 μg of fragmented FFPE DNA. Additionally, this product is also suitable for use with Yeasen Fragmentation Enzyme-based Library Preparation Kit. Features High Repair Efficiency: Provides higher repair efficiency for FFPE DNA.Streamlined Workflow: Simple operation with strong adaptability.High Compatibility: Enzyme components can be used with the end-repair and A-tailing system of library preparation kits, requiring only a single buffer to complete FFPE DNA repair and end-repair/dA-tailing. Applications Repair of damaged low-quality FFPE samples Library preparation for low-quality FFPE samples Specifications Cat.No. 12606ES24/ 12606ES96 Size 24 T /96 T Components Components No. Name 12606ES24 12606ES96 12606-A FFPE DNA Repair Mix 96 μL 384 μL 12606-B Endprep Buffer 2.0 144 μL 576 μL Shipping and Storage This product should be stored at -25~-15℃ for 1 year. Figures Figure1. The impact of FFPE repair reagents on library yield During the library construction process, two sets of experiments were conducted to compare the same samples. One group included a repair reagent in the end-repair and A-tailing step, while the other group did not. The experimental results showed that the FFPE repair reagent significantly increased library yield for low-quality FFPE samples by repairing the samples, whereas its effect on high-quality FFPE samples was less pronounced. Documents: Safety Data Sheet 12606_MSDS_HB250714_EN.PDF Manuals 12606_Manual_Ver.EN20260202.pdf Citations & References:
