dsDNA HS Assay Kit is a fast, sensitive and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. The kits include concentrated assay reagents, dilution buffer, and DNA standards. This kit is highly selective for dsDNA over RNA and is accurate for initial sample concentration from 10 pg/μL to 100 ng/μL. It is an ideal kit for DNA sample quantification such as NGS input DNA quantification and DNA library quantification. The kit has good tolerance to conventional contaminants such as salts, free nucleotides, solvents, detergents, and proteins. Features High Sensitivity: Maintains strong linearity across 0.2–100 ng. Excellent Stability: Consistent performance across production batches. Contaminant Tolerance: Resistant to interference from common impurities. Components Components No. Name Concentration 12642ES60 (100 T) 12642ES76 (500 T) 12640-A dsDNA Reagent 200×concentrate in DMSO 250 μL 1.25 mL 12640-B dsDNA Buffer Not applicable 50 mL 250 mL 12640-C dsDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL 12640-D dsDNA Standard 2 10 ng/μL in TE buffer 1 mL 5×1 mL Shipping and Storage This product should be stored at 2~8℃ away from light for 12 months. Application dsDNA quantification; DNA concentration determination Figures 1. Lot-to-lot Consistency: Stable over extended storage; valid for up to 2 years Figure 1. Good batch-to-batch consistency Real-time validity period: Batch 1-30 months, Batch 2- 27 months, Batch 3-22 months, all batches demonstrate long-term stability with minimal variation in shelf life. 2. Good linearity: 12640 has good linearity in the range of 0.2-100 ng. Figure 2. Linear Relationship Test Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12640. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA. 3. Dye binding specificity Figure 3. Specificity test Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested. Conclusion: Adding equal amounts of ssDNA slightly increases the 12640 reading; error
