HieffTM anti-exonuclease of Taq Antibody is a Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of exonuclease after binding to Taq DNA Polymerase at low temperature. HieffTM anti-exonuclease of Taq Antibody is denatured in the initial DNA denaturation step of PCR reaction, and the activity of exonuclease is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibody. Features Rapid enzyme activity release—Over 95% of enzyme activity can be released by heating at 95°C for 20 seconds. No Nuclease Contamination: Free from contamination of exonucleases, endonucleases, and RNases. Applications The concentration of this product is 5 mg/mL. 1 μL antibody could block 60-120 U the activity of exonuclease. It is recommended to mix the antibody and the Taq DNA polymerase at room temperature for 1 hour (incubate at room temperature for 2 hours when volume is greater than 200 mL, and customer should adjust the process when applied to larger volume), and then store at -20℃ overnight before use. Note: The specific activity of different Taq DNA Polymerase is variant, the blocking ratio need to be adjusted appropriately to achieve that the blocking efficiency is better than 95%. Specifications Classification Monoclonal Type Antibody Antigen Taq DNA Polymerase Form Liquid Components Name 31302ES60 （100 µg） 31302ES80 （1 mg） 31302ES90 （5 mg） HieffTM anti-exonuclease of Taq Antibody 20 μL 200 μL 1 mL Name 31302ES92 （10 mg） 31302ES93 （100 mg） 31302ES94 （1000 mg） HieffTM anti-exonuclease of Taq Antibody 2×1 mL 20 mL 200 mL Storage The product should be stored at -25°C~-15℃ for 2 years. Documents: Safety Data Sheet EN-31302-MSDS-Ver.EN20250207.pdf Manuals EN_31302_Manual_Ver.EN20250207.pdf
