ssDNA Assay Kit _12645ES

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ssDNA Assay Kit is a simple, sensitive, and accurate single-strand DNA (ssDNA) fluorescence quantitative detection kit with a good linear relationship between 1-200 ng. This kit co ntains a fluorescence detection reagent, buffer solution, and related ssDNA standards. Before use, dilute the buffer solution of fluorescence detection reagent into a working solution, and then add the ssDNA sample to be tested, then use the fluorescence microplate or Qubit Read with a fluorometer. The selectivity of this kit to single-stranded DNA is not higher than that to double-stranded DNA, but it has good tolerance to conventional pollutants such as proteins, salts, detergents, etc. Features Stable Signal: Combined with an exceptional dye and an upgraded, optimized buffer, it maintains stable fluorescence intensity at room temperature and exhibits strong applicability in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, or proteins, ensuring accurate quantification. High Sensitivity: Detection concentration as low as 50pg/μL. Rapid and Accurate: Features a simple operational workflow to achieve rapid quantification of oligonucleotides, primers, denatured DNA, and single-stranded circular DNA. Applications ssDNA quantification MGI Circular library and DNB quantification BS product quantification Specifications Assay ssDNA Quantitation Excitation/Emission 500/525 For Use With (Equipment) Qubit Fluorometer，fluorescence microplate No. of Reactions 100T/500T Product Line Qubit Quantitation Quantitation Range 1-200 ng, 50pg/μL - 200ng/μL Sample Volume 1 μL to 20 μL Detection Method Fluorescent Components Name Concentration 12646ES60 (100T) 12646ES60 (500T) ssDNA Reagent 200× in DMSO 250 μL 1250 μL ssDNA Buffer Not applicable 50 mL 250 mL ssDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL ssDNA Standard 2 20 ng/μL in TE buffer 1 mL 5×1 mL Shipping and Storage All the components are shipped with ice packs and can be stored at 2℃~8℃ away from light for 6 months. Please avoid repeated freeze-thawing. Figures Figure 1 Linearity PerformanceTest Sample: ssDNA standards; Conclusion: Good linearity within 0-200ng Figure 2 Comparison of measured values of different batches and original Thermophile productsThe stability of Yeasen（Cat # 12645）three batches of products is good, which can accurately quantify different types of ssDNA samples, and consistent with the competitive products(Thermo Q10212) Table 1 Good Contaminant Tolerance Documents: MSDS12645_MSDS_HB250211 Manuals 12645_Manual_HB20260401.pdf Protocol 12645-Qubit Microplate Reader Protocol-Ver.EN20260810 Show more