ssDNA Assay Kit is a simple, sensitive, and accurate single-strand DNA (ssDNA) fluorescence quantitative detection kit with a good linear relationship between 1-200 ng. This kit contains a fluorescence detection reagent, buffer solution, and related ssDNA standards. Before use, dilute the buffer solution of fluorescence detection reagent into a working solution, and then add the ssDNA sample to be tested, then use the fluorescence microplate or Qubit Read with a fluorometer. The selectivity of this kit to single-stranded DNA is not higher than that to double-stranded DNA, but it has good tolerance to conventional pollutants such as proteins, salts, detergents, etc. Features Fast and accurate ssDNA quantification High sensitivity, there is a good linear relationship in the low-concentration range Very suitable for the MGI platform library Applications ssDNA quantification MGI Circular library and DNB quantification BS product quantification Specifications Assay ssDNA Quantitation Excitation/Emission 500/525 For Use With (Equipment) Qubit Fluorometer，fluorescence microplate No. of Reactions 100T/500T Product Line Qubit Quantitation Quantitation Range 1-200 ng, 50pg/μL - 200ng/μL Sample Volume 1 μL to 20 μL Detection Method Fluorescent Components Name Concentration 12646ES60 (100T) 12646ES60 (500T) ssDNA Reagent 200× in DMSO 250 μL 1250 μL ssDNA Buffer Not applicable 50 mL 250 mL ssDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL ssDNA Standard 2 20 ng/μL in TE buffer 1 mL 5×1 mL Shipping and Storage All the components are shipped with ice packs and can be stored at 2℃~8℃ away from light for 6 months. Please avoid repeated freeze-thawing. Figures Figure 1 Linearity Performance Test Sample: ssDNA standards; Conclusion: Good linearity within 0-200ng Figure 2 Comparison of measured values of different batches and original Thermophile products The stability of Yeasen（Cat # 12645）three batches of products is good, which can accurately quantify different types of ssDNA samples, and consistent with the competitive products(Thermo Q10212) Documents: Manuals 12645_Manual_HB20260401.pdf MSDS 12645_MSDS_HB250211
