T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of a template and a primer. This enzyme has a 3'→5' exonuclease activity, but it does not have a 5'→3' exonuclease function. Applications 3´ overhang removal to form blunt ends 5´ overhang fill-in to form blunt ends Second strand synthesis in site-directed mutagenesis Probe labeling using replacement synthesis Cloning of PCR products without reliance on ligation reactions Specifications Source Bacteriophage T4 Molecular Weight 103 kDa Concentration 5 U/μL Unit Definition One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotide triphosphates (dNTPs) into a polynucleotide within 30 minutes at 37°C Components Components No. Name 12901ES60 12901ES76 12901ES90 12901ES08 12901-A T4 DNA Polymerase(5 U/μL) 20 μL 100 μL 400 μL 1 mL 12901-B 10 × Blue Buffer 100 μL 250 μL 500 μL 1.25 mL Shipping and Storage This product should be stored at -25 ~ -15℃ for 1 year. Documents: Safety Data Sheet 12901_MSDS_Ver.EN20241210.pdf Manuals 12901_Manual_Ver.EN20241210.pdf
