This product is suitable for multiple PCR experiments by two rounds of PCR amplication，and this kit is based on super multiplex PCR, combined with hot start modification technology and matched with the optimal buffer system, which can realize the re-amplification of Panel with panel multiplicity of 1-2,000. This product is compatible with the GC content of 25-75%. It has the characteristics of high uniformity, high specificity and high sensitivity. This reagent is suitable for multiple amplification of fragments with different lengths, such as 100-500 bp,and can be used for gel electrophoresis to identify multiplex products of fragments with different sizes within 20 pairs,and can also be used for the capture of amplicon more than thousand. Features Simplify operation: Packing the pre-mixed solution , and only need to add templates and primers in the experiment. Widely compatibility: compatible with microbial research, pathogen detection, genetic disease detection and agricultural detection etc. And Compatible with reverse transcription system, after reverse transcription, multiple amplification can be performed directly without purification. Excellent amplification performance: high detection rate, excellent specificity and good uniformity. Strict control of background bacteria: clean workshop, professional personnel and strict quality control. Applications Microbial detection；Multiplex PCR Specifications Cat.No. 13283ES03 / 13283ES08 / 13283ES50 Size 1 mL / 5×1 mL / 50 mL Components Components No. Name 13283ES03 13283ES08 13283ES50 13283 2× Multiplex PCR Mix 1 mL 5×1 mL 50 mL Figures Figure1. Yield of amplicon under Different Suppliers Libraries were prepared using Cat#13283 protocol ,from1 ng to 10 ng different samples(Fecal gDNA ,Soil DNA and Standard of Zymo Research #D6306). The results show that the performance of 13283 is better than other suppliers.
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