RT-LAMP Dye Assay Kit (UDG plus) contains fluorescent dye, Hieff™ Bst Plus DNA Polymerase, heat-resistant Hifair™ Ⅲ Reverse Transcriptase, MRI, and UDGas applied to LAMP reaction with RNA as template. This product is with high sensitivity and high specificity. Features High Sensitivity：50 copies/T, 25 μL system; High stability: stable at 37°C for 5 days with good stability, and can withstand repeated freezing and thawing more than 10 times; Specificity: 48-well NTC has no peaks at all, and the specificity is strong; Applications Rapid detection of pathogenic bacteria Rapid detection of pet pathogens On-site detection of animal husbandry Specifications Detection method Fluorescent dye method PCR method RT-LAMP Polymerase Hieff™ Bst Plus DNA Polymerase Type of sample RNA LOD 50 copies/T Specificity 100% Components Components No. Name 13762ES60 (100 T) 13762ES80 (1000 T) 13762-A Bst Buffer 600 µL 6 mL 13762-B Bst Enzyme Mix（UDG plus） 100 µL 1 mL Storage Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months.Part 2: 13762-B can be stored at -20°C for 6 months. Figures High Sensitivity Figure 1: Sensitivity Test Red: 50 copies/T, response less than 15 minutesFigure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%. Stability Figure 2.Stability testing at the same temperature Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days. Figure 3. -20℃ repeated freeze-thaw stability test Red: Repeat freeze-thaw 0 timesBlue: Repeat freeze-thaw 5 timesGreen: Repeat freeze-thaw 10 times Specificity Figure 4. 48-well NTC-specific assay 48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better. Related Blog： What is the role of Bst DNA polymerase in LAMP? High-quality Bst Enzyme Lyophilized Material Makes Molecular POCT More Convenient
