Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI Single End Barcode _13341ES

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Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and optimized Buffer si gnificantly reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard single-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents. Features Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.) Fast cyclization: a 1-hour rapid cyclization High cyclization efficiency: the cfDNA cyclization efficiency>30%,other sample types>40% Applications To prepare a single-stranded circular DNA library dedicated to the MGI high-throughput sequencer Specifications Product Type NGS Library Preparation Product Line DNA library Preparation Input DNA The recommended Input DNA is 1 pmol, if the PCR product is insufficient, down to 0.5 pmol For Use With (Application) Single Barcode DNA Cyclization Kit for MGI For Use With (Equipment) MGI Platforms Quantity 16/96 Reactions Components Components No. Name 13341ES16 (16T) 13341ES96 (96T) 13341-A Splint Oligo 96 μL 576 μL 13341-B Splint Buffer 240 μL 2×720 μL 13341-C Ligase 80 μL 480 μL 13341-D Digestion Buffer 128 μL 768 μL 13341-E Digestion Enzyme 32 μL 192 μL Figures Figure 1. Comparison of cyclization efficiency of PCR-free library preparation for different fragment lengths. Method: The PCR-free libraries were prepared with Cat#12202 PCR-free library prep kit.Results: The library cyclization efficiency of the different library fragments Cat#13341 was higher than that of Supplier M* Show more