Hifair™ V Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex quantitative PCR kit using RNA as the template. Reverse transcription and qPCR are performed in a single tube, simplifying the workflow and minimizing contamination risk. The kit uses a heat-stable Hifair™ V Reverse Transcriptase for efficient first-strand cDNA synthesis, and UNICON™ HotStart Taq DNA Polymerase for specific and robust amplification. It includes an optimized MP buffer with Mg²⁺, dNTPs, and additives that enhance specificity and multiplex amplification efficiency. A dUTP/UDG system is incorporated to eliminate carryover contamination from previous PCR reactions, ensuring reliable results. Features High sensitivity: The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL; Super stability: The product has passed real-time stability testing for 18 months; dUTP/UDG anti-pollution system: dUTP/ UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results; Support fast program: compatible with fast program, 30min can produce results. Components Components No. Name 13650ES50 (50 T) 13650ES60 (100 T) 13650ES80 (1,000 T) 13650ES92 (10,000 T) 13650-A 2×HifairTM V MP Buffer 750 μL 1.5 mL 15 mL 150 mL 13650-B HifairTM V Enzyme Mix 60 μL 120 μL 1.2 mL 12 mL [Note]: 1) 2×HifairTM V MP Buffer is the abbreviation of HifairTM V Multiplex One Step RT-qPCR Probe Buffer, which includes dNTPs, dUTP, Mg2+, stabilizers, enhancers, and more. 2) HifairTM V Enzyme Mix mainly contains heat-resistant HifairTM V reverse transcriptase, UNICONTM HotStart Taq DNA polymeras and UDGase. Shipping and Storage This product should be stored at -25~-15℃ for 18 months. Application RT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.) Figures 1. Excellent amplification performance Figure 1. Comparison of amplification performance of RT-qPCR kits from different brands Amplification of gradient samples using 13650 and similar products from other suppliers in respiratory and HFMD panels showed clear S-shaped amplification curves. The Ct values of 13650 were comparable to or earlier than those of other kits, indicating superior performance. 2. Compatible with regular and fast programs Figure 2. Test results of different reaction programs Detection of ORF and N genes of SARS-CoV-2 pseudovirus using 13650ES under both regular and rapid programs showed no difference in amplification curve peak shape or Ct values in the 4-plex system. 3. Stability Test Figure 3. Real-time stability testing(A) and Freeze-thaw stability(B) Documents: Safety Data Sheet 13650_MSDS_HB20250529_EN.PDF Manuals 13650_Manual_Ver.EN20241114.pdf
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