Hieff UNICON™ Hotstart E-Taq DNA Polymerase features an enhanced hot start Taq DNA polymerase, incorporating dual blocking antibodies. Both the 5'→3' polymerase and 5'→3' exonuclease activities of Taq DNA polymerase are effectively inhibited. A 30-second heating step at the pre-denaturation temperature serves to completely deactivate the antibodies, restoring DNA polymerase and exonuclease activities. This dual blocking capability not only safeguards against nonspecific amplification due to mismatch or primer dimer but also proficiently restrains the reduction in fluorescence signal caused by probe degradation. This ensures enhanced stability of the in vitro detection reagent during transportation or room temperature usage. Features High sensitivity effectively inhibit the non-specific amplification Applications Routine PCR amplification of DNA fragments Hotstart PCR Specifications Polymerase Taq DNA Polymerase Purity ≥ 95% (SDS-PAGE) Hot Start Built-In Hot Start Reaction Speed Standard Exonuclease Activity 5' - 3' Components Components No. Name 13824ES72(250 U) 13824ES76(500 U) 13824ES80(1000 U) 13824ES92(10000 U) 13824ES93(25000 U) 13824 Hotstart E-Taq DNA Polymerase, 20 U/μL 12.5 μL 25 μL 50 μL 500 μL 1.25 mL Shipping and Storage The product is shipped with dry ice and can be stored at -15°C~-25°C for 2 years. Documents: Safety Data Sheet 13824_MSDS_HB250826_EN.PDF Manuals 13824_Manual_Ver.EN20250904.pdf