FuniCut™ series endonucleases are fast restriction enzymes developed through genetic engineering that can precisely cut DNA within 5–15 minutes. These enzymes are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. All FuniCut™ fast restriction enzymes share the same digestion buffer, which simplifies reaction setup. Additionally, they have a high enzyme activity margin, making them capable of easily handling excess substrate or challenging templates. Specifications Cat. No. 15010ES78 Size 600 T Recognition Site 5'-G↓AATTC-3' 3'-CTTAA↑G-5' Recommended Reaction Conditions 1× FuniCut™ Buffer; incubate at 37°C Enzyme activity 20 U/μL Inactivation Conditions Incubate at 80°C for 20 minutes. Cohesive-end ligase FunII Components Components No. Name 15010ES78 15010-A FuniCut™ EcoRI 600 μL 15010-B 10×FuniCut™ Buffer 3×1 mL 15010-C 10×FuniCut™ Color Buffer* 3×1 mL 【Note】: 10× FuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
